Fig. 2. Localization of dynamin and ß-adrenergic receptor (ßAR) immunoanalogue in isoproterenol-treated and untreated Paramecium cells using confocal laser scanning microscopy. Dual fluorochrome immunolabeling was performed as described in Materials and methods. Colocalization (yielding a yellow orange image) of ßAR (green) and dynamin (red) inside the cell in small punctate accumulations (arrows) was observed in the overlay of the series of confocal sections performed at 0.6 µm intervals (A–D). Such a pattern of colocalization was not observed in the untreated cells (E). Bar, 16 µm.