Fig. 1. Effect of casein-based diet containing low (filled square), normal (filled diamond) and high (filled circle) levels of dietary Fe for 8 weeks (measured Fe in diet, 33, 175 and 1975 mg kg^-1, respectively) on tissue Fe concentration in the intestine (A), stomach (B), liver (C), serum (D), muscle (E) and gill (F) of rainbow trout. Values are means ± S.E.M. (N=9 fish per time point). Different letters (a, b or c) denote statistically significant differences in tissue Fe concentration within each time point between experimental dietary Fe treatments (ANOVA followed by LSD test, P<0.05). All data are denoted the letter a (not shown for clarity in figures) unless otherwise indicated. Thus, b indicates the high-Fe diet is different from either low or normal diets in the panels for Fig. 1, but low and normal diets are not different from each other. No treatment differences were observed in muscle or gill. Asterisk indicates a significant difference between initial values (open symbol) in stock fish fed normal commercial trout food compared with fish at week 2 on the normal Fe diet (Student's t-test, P<0.05, N=9).