Fig. 3. Experiment 1, showing the test performance of individually assayed wild-type CS-F larvae, which had received either of two reciprocal training regimes: Light-/Dark+ (filled symbols) or Light+/Dark-(open symbols). (A) Percentage of animals located in a covered ('dark') quadrant of an X-plate photoresponse assay. Animals were observed every 10 s for 5 min. The 50% line indicates random distribution. (B) For each animal, a PREF value was calculated. Positive values indicate dark preference and negative values, light preference. The box plot represents the median as the middle line and 10 and 90 and 25 and 75% quantiles as whiskers and box boundaries, respectively. (C) A learning index (LI) was calculated for each pair of animals which underwent either Light-/Dark+ or Light+/Dark-training by subtracting the animals PREF values and dividing the result by two. Positive LIs indicate associative learning. The box plot represents the median as the middle line and 10 and 90, and 25 and 75% quantiles as whiskers and box boundaries, respectively. ^*P<0.0001 (significantly above chance level). Sample sizes are N=75, 75 for filled and open symbols, respectively; N_LI=75.