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Fig. 5. Tissue distribution of rainbow trout glutamine synthetase (GS) using high-stringency northern analysis. Membranes were probed using 32P-labelled random-primed cDNA probes for four GSase genes (Onmy-GS01, Onmy-GS02, Onmy-GS03 and Onmy-GS04) and ß-actin, and hybridized at 65°C. Each lane contained 10 µg of total RNA. K, kidney; H, heart; L, liver; G, gill; Int, intestine; W, white muscle; R, red muscle; B, brain. Arrows indicate the location of the respective band.