Fig. 3. Laser ablation to the anterior and posterior pigment cells in the sensory vesicle of the larvae. (A) Trunk of the larva, showing the anterior (otolith) and posterior (ocellus) pigment cells. (B) When the stalk of the otolith (arrowhead) is ablated by laser, the anterior pigment cell (C; arrow) is detached from the wall in the lumen of the sensory vesicle and is thus defocused. (D) The laser is focused onto the center of the posterior pigment cell (arrowhead), making a hole was made in the center of the posterior pigment cell (arrowhead) (E). Bars, 50 µm (A); 20 µm (B—D).