Fig. 2. FITC-inulin distribution in molting larvae. FITC-inulin was injected into the molting fluid (MF) of molting larvae. All larvae were exposed to the dye for 10 h before they were dissected. Foreguts are shown in the upper panels, and midguts are shown in the lower panels. (A) An early-molt larva [13–16 h after head capsule slippage (HCS)] with low levels of fluorescent material in its digestive tract. (B) We observed high levels of FITC-inulin in the foregut and midgut of the late-molt larva (22–25 h after HCS). The robust contractions of the foregut had resumed by the late-molt stage. (C) A late-molt stage larva (22–25 h after HCS) whose foregut was silenced by lesioning the frontal ganglion (FG) shows only low levels of fluorescent material in its gut. All larvae were photographed with the same camera exposure and microscope settings. Arrows indicate the right edge of the brain. Scale bar, 500 µm.