Fig. 2. MAPK responses in stress-tolerant animals. (A) JNK activity in organs from control (C) and anoxia-exposed (1, 5 and 20 h anoxic submergence at 5°C) hatchling turtles Trachemys scripta elegans. Left, representative autoradiograms of phosphorylated ³²P-c-Jun-GST; right densitometry results (means ± S.E.M.) for N=3 trials. (B) Amount of phosphorylated (active) p38 in organs from control (C) versus frozen (20 min, 1 and 12 h at –2.5°C) wood frogs Rana sylvatica. On the left are representative western blots using antibody to the phosphorylated (tyr 182) peptide; on the right are densitometry results, means ± S.E.M., N=3. *Significantly different from the corresponding control value, P<0.05. From Greenway and Storey (1999, 2000a).