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Fig. 7. Changes in hemolymph Na+ (A) and Cl- (B) concentrations of eyestalkless Astacus leptodactylus 8 h and 24 h following injection of high-performance liquid chromatography (HPLC) fractions (10 SGequiv) corresponding to zones Z1 (N=8), Z2 (N=8) and Z3 (N=8) (see Fig. 4A). Each column represents the mean ± S.E.M. of the increase in ion concentration compared with the control. Statistical comparison with controls injected with a Van Harreveld saline (N=6): *P<0.05; **P<0.01; ***P<0.005.