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Fig. 6. Changes in osmolality of eyestalkless Astacus leptodactylus 8 h and 24 h following injection of high-performance liquid chromatography (HPLC) fractions (10 SGequiv) corresponding to zones Z1 (N=8), Z2 (N=8) and Z3 (N=8) (see Fig. 4A). Each column represents the mean ± S.E.M. of the increase in hemolymph osmolality compared with the control. Statistical comparison with controls injected with a Van Harreveld saline (N=6): *P<0.05.