JEB -- Morton and Anderson 206 (6): 937 Figure IG3

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Fig. 3. Heterodimerization of MsGC-ß3 with MsGC- ${alpha}$ 1 and MsGC-ß1. Extracts of COS-7 cells that were transiently cotransfected with MsGC-ß3 and either empty vector (pcDNA3.1), or hexa-histidine (His₆)-tagged MsGC-ß3 ${Delta}$ C338, MsGC- ${alpha}$ 1 or MsGC-ß1, were incubated with nickel-chelated agarose and the bound proteins analyzed by western blot. (A) Blot probed with MsGC-ß3 antiserum shows that MsGC-ß3 is pelleted when cotransfected with either tagged MsGC-ß3 ${Delta}$ C338, tagged MsGC- ${alpha}$ 1 or tagged MsGC-ß1, but the intensity of the immunoreactivity is highest when cotransfected with tagged MsGC-ß3 ${Delta}$ C338. (B) Western blot of input extract stained with anti-MsGC-ß3.