JEB -- Morita et al. 206 (5): 913 Figure IG3

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Fig. 3. Effect of (A) cAMP, cGMP and Ca²⁺, and (B) various concentrations of Ca²⁺ on demembranated sperm motility. Demembranated sperm were suspended in reactivation solutions containing 220 µmoll^-1 Mg-ATP²⁺, 175 mmoll^-1 potassium acetate, 1 mmoll^-1 free Mg²⁺, 1 mmoll^-1 DTT, 0.5 mmoll^-1 EGTA, 0.5 mmoll^-1 EDTA, 20 mmoll^-1 Hepes-NaOH (pH 8.0). (A) Solutions contained 10 µmoll^-1 cAMP, 10 µmoll^-1 cGMP, 10^-4 moll^-1 free Ca²⁺ or reactivation solution only (ATP). (B) Effect of [Ca²⁺] on reactivation of sperm motility. Values are means ± S.D.; N=5 (A); N=7 (B).