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Fig. 5. Western blot analysis for vacuolar-type proton pump (V-ATPase) protein expressed in the gills of killifish adapted to a low-NaCl environment. The membranes were incubated with anti-V-ATPase (lane B) and antibody pre-incubated with the antigen (lane A). Positions of molecular markers (kDa) are indicated on the left side of the figure. Two specific protein bands (arrows) were obtained.