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Fig. 3. Dose—response curves of various inhibitors on water intake by seawater eels. Water intake was measured during a 20 min period after administration of the inhibitor, and is expressed as % of control rate measured during a 20 min period before administration (100%). Ghrelin (filled squares), eel atrial natriuretic peptide (eANP; filled circles), serotonin (5-HT; open circles), phenylephrine (filled diamonds), prolactin (PRL; open triangles) and {gamma}-amino butyric acid (GABA; filled triangles) were injected intracranially at a rate of 1.25 µl min-1 for 4 min. Values are means ± S.E.M. Number of experiments is indicated in parentheses. *P<0.05, **P<0.001, compared with the control value (paired t-test).