Fig. 1. Transfer of Paramecium to ß,
-methylene ATP (met-ATP)
causes immediate avoiding reactions (AR). Digital video imaging shows the
swimming path of a cell to be relatively straight in the control solution (A),
while a different cell in 25 µmol l-1 ß,
-methylene
ATP shows repetitive jerking back and forth, indicative of AR (B). The video
capture rate was approximately 16 frames s-1. (C) The percentage of
cells showing AR (% AR) increased in a concentration-dependent manner, with
100% of the cells showing AR at concentrations of ß,
-methylene ATP
above 25 µmol l-1 and an EC50 of approximately 12
µmol l-1. Each point represents the mean ± S.D. of three
trials.