Fig. 6. Footprinting of the H-strand promoter region using different primers. The
control region 5' to the 12S rRNA was footprinted using the primers HSP
3, HSP 4 and HSP 5 for panels AC, respectively. With naked DNA as a
reference, undermethylated residues are noted by unfilled squares and
overmethylated residues are noted by filled squares. Lane assignments were as
follows. Panels A and B: 1, in organello anoxia, pH 6.4; 1a, in
organello anoxia, pH 6.4; 2, normoxia, pH 6.4; 3, in organello
anoxia, pH 7.9; 4, in organello control (normoxia, pH 7.9); 5, naked
DNA. Panel C: 1, normoxia, pH 6.4; 2, in organello anoxia, pH 6.4; 3,
in organello control (normoxia, pH 7.9); 4, naked DNA. Genomic
positions are indicated by arrows.