Fig. 9. Western blot analysis of Ha-CalpM in claw muscles during the moulting cycle. Supernatant fractions (32 µg protein) of claw muscles at different moulting stages were electrophoresed on 6% SDS-polyacrylamide gels and either stained with Coomassie Blue (B) or transferred onto PVDF membrane and probed with Ha-CalpM antiserum (A) or pre-immune serum (data not shown). The 62 kDa isoform (arrow) was present at all moulting stages: intermoult (lanes a,b), premoult (lanes c,d), and postmoult (lanes e,f). Proteins binding non-specifically to the antibody are indicated by asterisks in A. Positions of molecular mass standards (kDa) are indicated. I-1 and I-2, intermoult stage at 20% and 30% of the intermoult interval, respectively; D1, premoult stage D₁; D3, premoult stage D₃; P-1 and P-2, postmoult stage at 7% and 13% of the intermoult interval, respectively.