Fig. 8. Real-time PCR analysis of Ha-CalpM expression in lobster crusher claw muscles during the moulting cycle. Total RNA from intermoult, early premoult (stage D₀), and early postmoult (2-4 days post-ecdysis) lobsters was DNase-treated, reverse-transcribed, and PCR-amplified in reactions containing SYBR Green I fluorescent dye (see Materials and methods). Values are means ±1 S.D. (N=4 for each moult stage). Ha-CalpM transcript copy number did not change significantly (P<0.28; ANOV A single factor analysis).