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Fig. 5. RT-PCR analysis of Ha-CalpM and {alpha}-actin expression in lobster tissues. Total RNA from various tissues were DNase-treated, reverse-transcribed, and PCR-amplified using primers directed to the 5' ends of Ha-CalpM (bp 1-18 and 600-619 in Fig. 1) and Ha-Actin1 (bp 1-23 and 379-400; see Koenders et al., 2002). PCR amplification of 18S rRNA served as an internal standard. (A) The PCR products synthesized from Ha-CalpM (619 bp) and 18S rRNA (324 bp). (B) The PCR product (400 bp) synthesized from {alpha}-actin. Shown are negative images of ethidium bromide-stained agarose gels. There were high amounts of Ha-CalpM mRNA in cutter claw, crusher claw and deep abdominal muscles (lanes a—c). Little or no Ha-CalpM product was amplified from other tissues (lane e—i). {alpha}-Actin was expressed in all tissues except antennal gland (lane i). Abbreviations as in Fig. 4.