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Fig. 3. Phylogenetic relationship of Ha-CalpM to other calpains. Amino acid sequences of the proteinase domain (domain II) of calpains from selected species were compared using the Clustal V method (see Materials and methods). Branch lengths are proportional to the inferred phylogenetic distances, with the Shistosoma mansoni domain II sequence serving as the outgroup. Ha-CalpM was grouped with Drosophila calpains (Dm-CalpA and Dm-CalpB), reflecting the high sequence identity among the three sequences. GenBank accession numbers for Drosophila calpains and human m-calpain are given in the legend of Fig. 2; the accession numbers for the other calpains are X92523 for human p94 (CAPN3), AF022799 for mouse nCL-4 (CAPN9), M74233 for S. mansoni, Y10656 for mouse calpain 5 (CAPN5), and U14480 for C. elegans tra-3.