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Fig. 1. The complete sequence of Ha-CalpM cDNA cloned from a lobster fast muscle library. The sequence (1977 bp) contains an open reading frame (bp 16-1743) encoding a protein of 575 amino acids (aa) with a predicted mass of 66.3 kDa. The three conserved aa (Cys 141, His 299, Asn 329) essential for hydrolase activity are indicated in bold. A stretch of 17 aspartates (aa 454-470) is underlined. The numbers on the left indicate aa positions; those on the right indicate nucleotides. The highly conserved aa sequences used for initial cloning with nested PCR are underlined and italicized. GenBank accession no. AY124009.