Fig. 1. Relative distribution of isolated gill epithelial cells. Cells were separated into different fractions using a discontinuous Percoll density gradient, peanut lectin agglutinin (PNA) binding and magnetic separation (see Materials and methods). Insert shows the numbers of the PNA^- and PNA⁺ cells found within the 1.05-1.09 g ml^-1 density interface layers expressed as a percentage of the total epithelial cells harvested. Data represent means + 1 S.E.M. (N=4). Means with different letters indicate that they are significantly different (P0.05).