Fig. 2. Temperatures of crystallization (T_c) of Chrysemys picta, reared on vermiculite and acclimated to 4°C, after ingesting a small amount of autoclaved nesting soil or a washing of non-autoclaved nesting soil. Control turtles ingested vehicle (water) or nothing (sham-fed). Turtles were tested 24 h or 33 days after treatment. Mean values (± S.E.M., N=5 replicates per group) identified by different letters are statistically different (ANOVA/Student—Newman—Keuls multiple comparisons test; P<0.05).