Fig. 2. Determination of specificity of the antibodies by western blot analysis with non-purified bacterially expressed eel vacuolar ATPase subunit B isoforms vatB1 and vatB2. 30 µg protein were used for each lane. (A) Western blot probed with antibody #1035, which is specific for vatB1: left lane, vacuolar ATPase B subunit isoform vatB1 (kidney isoform); right lane, vacuolar ATPase B subunit isoform vatB2 (brain isoform). (B) Western blot probed with antibody #1034, which recognizes both isoforms of the B subunit. Without primary antibody, no bands could be detected.