Fig. 2. SPaSE in males subjected to the four different conditions. (A-D) Percentage
of males exhibiting auto-spermatophore extrusion (aSE; above) or the calling
song (CS; below). (A) Free-moving condition. Note the very low percentage at
50-70 min of males exhibiting auto-spermatophore extrusion (aSE) compared to
males exhibiting the calling song to a female (CS). (B) Restrained condition.
(C) Semi-dissected condition. (D) Dissected condition. The columns at >120
min indicate the percentage of males that did not show auto-spermatophore
extrusion within 120 min. Op. (C,D) indicates the period of the 10 min
operation immediately after spermatophore preparation. M, median. These
conventions are also used in Figs
3 and
10.