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Fig. 7. Gill Na+/K+-ATPase (A) {alpha}1a and (B) {alpha}1b mRNA in trout held in freshwater (Pre) and following transfer to freshwater (filled circles), 40% seawater (open squares) and 80% seawater (filled triangles). mRNA expression is normalized to the control gene, EF-1{alpha}, and all data following the transfer are expressed relative to the pre-transfer freshwater gill samples. Vertical broken line represents the abrupt transfer. Symbols are offset for clarity where necessary. Asterisks indicate significant difference from the control (freshwater) value.