Fig. 4. Effect of hyperoxia on lens size. Beginning on E7, lenses were incubated in 50% O₂:50% N₂. Vibratome slices were prepared from hyperoxic or normoxic lenses and stained with acridine orange. (A) Lens diameter (D) is consistently larger in hyperoxic lenses (open circles) compared with normoxic controls (closed circles). (B) Lens axial thickness (T) is greater in hyperoxic lenses than in normoxic controls, and this difference reaches statistical significance by E17. (C) The area of mid-sagittal slices prepared from hyperoxic lenses is greater than normoxic controls. Asterisks denote significant (P<0.05) difference between hyperoxic lenses and age-matched controls.