Fig. 6. Expression of the prodomain transgene and the endogenous myostatin gene in skeletal muscles of adult transgenic and non-transgenic zebrafish. For each sample, RT-PCR was performed using three different sets of primers that are specific for the transgene (mylc-MSTN^pro), the endogenous myostatin gene and the transgene (Total MSTN), and the elongation factor 1- (Ef-1) mRNA transcripts, respectively. Lane 1, RT-PCR results using mylc-myostatin^pro plasmid DNA as positive control. Lanes 2 and 3, RTPCR results showing the expression of the endogenous gene (total MSTN) and EF-1 in the skeletal muscle of two individual non-transgenic fish (2 and 3). Lanes 4 and 5, RT-PCR results showing the expression of the Myostatin prodomain transgene (mylc-MSTN^pro), the endogenous myostatin gene and the transgene (total MSTN) and the EF-1. Lane 6, RT minus control of non-transgenic fish. Lane 7, RT minus control of transgenic fish.