Fig. 2. Innervation of the ovotestis (OT) in Helix aspersa. (A) Wholemount of the OT embedded within the digestive gland. The dotted line delineates the perimeter of the OT. Neurobiotin was applied to the cut end of the intestinal nerve in this preparation (note the darkened stump), and the oocytes reacted nonspecifically to the histochemical procedure used to visualize the nerve. (B) Magnification of a portion of the hermaphroditic duct and OT from A showing fine branching in the intestinal nerve. (C) Frozen section (20 µm) from a second preparation showing innervation (arrows) along the wall of an acinus. Scale bars: A, 1 mm; B, 550 µm; C, 170 µm.