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Fig. 4. Maps of recombinant plasmids and strategy for constructing a strain of Bacillus thuringiensis that produces Cyt1A, Cry11B and the Bacillus sphaericus 2362 binary toxin. (A) p45S1 containing cyt1A from B. thuringiensis subsp. israelensis and binary toxin gene from B. sphaericus 2362. (B) pPFT11Bs-CRP containing cry11B from B. thuringiensis subsp. jegathesan. Amp, ampicillin-resistant gene; Erm, erythromycin-resistant gene; Cm, chloramphenicol-resistant gene; cyt1A-p, cyt1A promoters; cry1Ac-p, cry1Ac promoters; E. c. ori, E. coli replication origin; B. t. ori, B. thuringiensis replication origin.