Fig. 2. Immunolocalization of trypsin-modulating oostatic factor (TMOF) binding to mosquito midgut receptor 48 h after the blood meal. (A) A transverse section (7 µm) was incubated with TMOF and polyclonal antibodies. Distinct binding of TMOF to its receptor was observed on a small area of the transverse section (dark area between arrows). (B). Fourfold magnification of the binding region. PM, peritrophic membrane; Lu, gut lumen; He, hemolymph side of the gut.