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Fig. 5. Immunolocalization of the AaeCG12773 cotransporter in the mosquito digestive system. (A) The gut and Malpighian tubules of a 4th instar larva of Aedes aegypti. Specific immunostaining is detected in gastric ceca (CA), anterior (AMG) and posterior (PMG) midgut and pylorus (P). Immunofluorescence in the Malpighian tubules (MT) and rectum (R) is low and does not exceed background levels of pre-immune serum. (B,C) Immunohistochemistry of sections of a 4th instar A. aegypti larva. Subcellular localization of the AaeCG12773 cotransporter by specific antibodies clearly shows that it is predominantly present in the basolateral membrane of the midgut. No specific immunoreactivity was observed in sectioned Malpighian tubules and rectum.