Fig. 2. (A) The structure of plasmid Hermes QH7011 used to genetically transform Aedes aegypti. The plasmid pBSKS contains an autonomous Hermes element with the Hermes transposase gene under the control of the hsp70 promoter of Drosophila melanogaster as well as EGFP (enhanced green fluorescent protein) under the control of the D. melanogaster actin5C promoter (not drawn to scale). M. domestica genomic DNA flanking the ends of Hermes are relics of the original cloning of Hermes and are indicated by boxes. (B) Structure of Hermes QH7011 in the germ line of A. aegypti as deduced by Southern blots and PCR analysis of the breakpoints (data not shown). The entire element has integrated along with the Musca flanking sequences and the pBSKS vector DNA. Rearrangements towards the ends of the entire integrated sequence are shown and consist of a partial duplication of the Musca sequences flanking the right end. In addition, a rearrangement of pBSKS vector DNA in the form of an inversion occurred during the integration process (broken line).