Fig. 6. Size-class-specific changes in amount of poly(A)⁺ tail (PAT) over time as a function of in organello treatment. Mitochondria were incubated under the indicated conditions, RNA extracted and PAT analysis performed. Increases represent the ratio of PAT amounts present after 6 h compared to 1 h in the size classes indicated. (A-D) The four mRNA species studied: CYB (A); COXI (B); ND1 (C); ATP6 (D). Asterisks indicate significant differences from controls. Values without error bars are data taken directly from gels shown in Fig. 5; values with error bars represent mean changes in total polyadenylation from 1-6 h for N=3 separate experiments.