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Fig. 7. Analysis of myosin heavy chain (MHC), actin and P75 expression in slow fibers by RT-PCR. Experimental arrangement is as in Fig. 6. Different slow fibers [distal cutter (lane a), ventral cutter (lane b), crusher (lane c), lateral superficial extensor (lane d)] predominantly expressed S1 MHC and actin. The distal cutter (lane a) shows low amounts of fast MHC product. Actin expression (B) with this method appears to be higher than S1 MHC expression (A). (Inverse images of ethidium bromide-stained gels.)