Fig. 3. (A) Western blot analysis of Na⁺K⁺-ATPase. Proteins of molecular mass approx. 115 kDa were probed with anti-chicken Na⁺K⁺-ATPase antibody in the plume and in the trophosome membrane fractions (S3) but not in the soluble fractions (S2). (B) Western blot analysis of V-H⁺ATPase. Two bands of approximately 59 and 57 kDa were visualized with anti-yeast V-H⁺ATPase antibody for the plume fraction, but only one band of 57 kDa in the trophosome. The positions of marker proteins (kDa) are shown.