Fig. 6. MAP is highly detected in the gonad-duct wall of adult males. (A) One of the transversal gonadal ducts (TGDs; arrows) located in the posterior mantle region was microsurgically isolated and assayed as described in Materials and methods (bar, 0.4 cm). (B) Western blot (after SDS-PAGE) with anti-MAP antibodies. The protein loading for each lane is indicated. Lane 1, extract from the total male gonad/mantle (12 µg). Lanes 2 and 3, extract from the gonad-duct wall (12 and 6 µg, respectively). Lane 4, cell-free luminal fluid (5 µg). Lane 5, sperm cells from the lumen (10 µg). Note that the intensity of MAP imunostaining is higher in the gonad-duct wall (Lanes 2, 3) in comparison with that of the luminal fluid (Lane 4) and sperm (Lane 5).