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Fig. 2. (A) Full-length (122 kDa) Acp36DE and its 68 kDa processing product in the seminal receptacles (top) or spermathecae (bottom) of 90 females at 0.33 h or 2 h after the start of mating to wild-type males or to males transferring seminal fluids but no sperm. A cross-reactive protein band at 75 kDa serves as a loading control. Results were similar in repeats of this experiment. (B) Full-length (122 kDa) and processed (68 kDa; Bertram et al., 1996) Acp36DE in the seminal receptacle and spermathecae of 90 females mated with wild-type males 0.17 h or 1 h after the start of mating.