Fig. 4. Myomesin isoforms are differentially regulated in muscle classes during development. qPCR analysis of hindlimb, cardiac and extraocular muscle (EOM) was performed with the Roche LightCycler and SYBR green reagent. Different primer sets were used to evaluate expression levels of (A) myomesin 1, (B) EH-myomesin 1 and (C) myomesin 2 in the three muscle groups between the ages of E18 and P45. Data establish differential regulation patterns for each transcript and muscle group during development.