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Fig. 4. Cumulative changes in pHi ({Delta}pH) and in proton load ({Delta}P) due to changes in [PCr] (expressed in µmol g-1 wet mass) at (A) 15°C and (B) 25°C. The data are related to the different experimental pHe (black, 7.9; white, 7.3; grey, 7.0). Point zero (the origin of the axis) corresponds to the values registered at the beginning of the experiments. The diagonal lines through the origin, calculated in the range where the change in pH was due solely to changes in [PCr], have a slope equal to -1/ß (see text).