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Fig. 3. The production of cAMP (pmoles 10-6 cells) in alveolar type II cells isolated from warm-active and torpid ground squirrels and assayed at two assay temperatures (37°C and 4°C). cAMP production was calculated using a direct cAMP ELISA kit (Amersham Pharmacia, Sydney, Australia). Bars represent the amount of cAMP produced (mean ± S.E.M.) in N=5 experiments in all cases. *Significant increase above basal cAMP production for each experimental group; {dagger}significant difference between warm-active cells assayed at 37°C and torpid cells assayed at 4°C (t-test, P<0.05).