Fig. 6. Phosphorylation of MAPKAPK2 by 30 µmol l^-1 H₂O₂. (A) Protein (100 µg) from control hearts (C), hearts perfused with 0.5 mol l^-1 sorbitol for 15 min (S), hearts perfused with 30 µmol l^-1 H₂O₂ in the absence of inhibitor for the times indicated or the presence of 1 µmol l^-1 SB203580 for 2 min (I) was assessed by immunoblot using phosphospecific (top panel) or total (bottom panel) anti-MAPKAPK2 antibodies. Blots shown are representative of three independent experiments. (B) Densitometric analysis of phospho-MAPKAPK2 bands by laser scanning. Results are means ± S.E.M. for three independent experiments. ^**P<0.01 vs control value.