Fig. 3. Time course of JNKs activation by 100 µmol l^-1 H₂O₂. Protein (100 µg) from hearts perfused in the absence (C) or presence of 100 µmol l^-1 H₂O₂ for the times indicated was assessed by immunoblot using phosphospecific anti-JNKs antibody (A, top panel) or total anti-JNKs antibody (B). Blots are representative of three independent experiments. The bottom panel in A shows quantification of JNK1 and JNK2 bands by laser scanning densitometry. Results are means ± S.E.M. for three independent experiments. ^*P<0.05, P<0.001 vs control value.