Fig. 1. Isoelectric focusing of erythrolysates of (A) Symenchelis parasitica and (B) Thermarces cerberus (described in Materials and methods). Circles, absorption at 540 nm; triangles, pH values at 22°C; solid rectangles, Hb-containing fractions that were pooled and dialysed for Hb–O₂ binding studies; shaded rectangles, fractions that contained greyish precipitated material. I, II and III refer to hemoglobins (Hbs) I, II and III, respectively.