Fig. 3. Confirmation of cone identity by in situ hybridization using riboprobes against ultraviolet-sensitive (UVS) and short-wavelength-sensitive (SWS) opsins. (A) SWS opsin mRNA appears only in the central single cones. (B,C) UVS opsin mRNA appears only in the corner single cones. Arrowheads point to the partitioning membrane of the double cones. The partitioning membrane points to the central single (SWS) cone. A and B are 10 µm cryosections of retina in a tangential plane, and in situ labelling is represented by a dark precipitate. C is whole-mount retina in a tangential view, with fluorescent labelling of riboprobe. Scale bars, 10 µm.