Fig. 6. AFD-killed worms avoid warm temperature. (A) AFD neurons of N2 (CGC) were irradiated during the L1 stage. After worms were grown to adults at 20°C, population distribution on a temperature gradient was examined. Results with 48 AFD-killed worms (combined from four experiments; filled circles) are shown with 51 control N2 animals (simultaneously assayed in three experiments; open circles). (B) AFD neurons of N2 carrying an AFD marker, the gcy-8::gfp fusion gene, were irradiated in the L1 stage and, after the worms had grown to adults, movement on a temperature gradient was observed. Means of instantaneous velocities (filled circles) and their elements in gradient directions (open circles; positive values indicate upward movement; negative values indicate downwards movement) are shown in the left-hand panel (N=1178 intervals). Examples of traces are shown in the right-hand panel.