Fig. 2. (A) Representative western blot showing varying amounts of eIF2–FLAG(P) using the immunoblot procedure described in the Materials and methods. The film of the immunoblot was scanned, and densitometric analysis was performed using the program NIH Image 1.62. (B) Representative standard curve of density versus amount of eIF2–FLAG(P). Similar standards were run on all western blots to aid quantification.