Fig. 7. Western analysis of adult Ae. aegypti. Membrane and cytosolic fractions separated from head (lanes 1, 2, 7, 8), thorax (lanes 3, 4, 9, 10) and abdomen (Abd., lanes 5, 6, 11, 12) of adult Ae. aegypti were probed with affinity-purified -AeaEAAT antibody (lanes 1–6) or with the -AeaEAAT antibody that had been preadsorbed with the antigenic peptide (10 µmol l^-1) (lanes 7–12). Odd-numbered lanes correspond to membrane fractions, and even-numbered lanes to cytosolic fractions. The -AeaEAAT specific band migrates at a molecular mass of 50–52 kDa (arrowheads in lanes 1, 3, 5). Arrowheads in lanes 7, 9 and 11 indicate the missing band upon peptide preadsorption. The positions of molecular mass (kDa) proteins are shown.