Fig. 4. Amino acid inhibition of D-aspartate transport. The ability of
the 20 amino acids (each at 5 mmol l-1) to compete the transport of
D-aspartate (as a mixture of 1 µmol l-1 cold and 30
nmol l-1 3H-D-aspartate) was examined. Values are a mean
of triplicate experiments, plotted as a percentage of D-aspartate
transport (fmol cell-1 h-1) in the absence of any
competing amino acid. Asterisks indicate significant inhibition at the 99%
confidence interval. Note that the results for tyrosine (Y) and tryptophan (W)
also appeared to give significant inhibition, but are not indicated by
asterisks due to the acidic conditions required for dissolving these amino
acids at 5 mmol l-1, which skewed the results. Amino acids are
indicated by the single letter code. , control (no competing amino
acid).