Fig. 1. Molecular identification of the V-type H⁺-ATPase in Malpighian tubules of the yellow fever mosquito. (A) SDS–PAGE: lane 1, standard molecular mass proteins; lane 2, holoenzyme of the V-type H⁺-ATPase of Manduca sexta; lane 3, crude tubule extract of Malpighian tubules of Aedes aegypti. (B) Western blot: antibodies raised against the V-type H⁺-ATPase of Manduca sexta recognized similar proteins in extracts of Malpighian tubules of Aedes aegypti. Lanes 1, 3 and 5 are the Manduca holoenzyme, and lanes 2, 4 and 6 are the crude extracts of Aedes Malpighian tubules. Ab 353-2, Ab 488-1 and Ab C23 are antibodies against the V₁ complex, C subunit and B subunit of the proton pump, respectively.