Fig. 5. Immunostaining of midbrain and retinal sections of control (A,C) and bulbectomized (B,D) zebrafish. (A,B) FMRFamide staining was seen in the midbrain of control (A, arrows) but not bulbectomized fish (B). (C,D) Double-labeled retinal sections of control (C) and bulbectomized (D) zebrafish with antibodies against FMRFamide and tyrosine hydroxylase identifying terminal nerve (TN) axons (red) and dopaminergic interplexiform cells (DA-IPCs) (green), respectively. Note the absence of FMRFamide staining in bulbectomized zebrafish. Scale bar, approximately 350 µm (A,B), 35 µm (C,D).